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New research published June 3, 2026 in Nature shows that the waxed chambers where honeybee queens develop — not just royal jelly — play a critical role in queen formation.
Scientists led by researchers at the University of California, Riverside and collaborators compared peanut‑shaped queen cells with ordinary worker comb in western (Apis mellifera) and eastern (Apis cerana) honeybees.
They found queen‑cell wax is physically and chemically distinct: softer, less dense and containing different fatty acids and scent compounds.
A previously unrecognized class of young “queen cell builder” workers appears specialized for constructing these nurseries, maintaining higher body temperatures and distinct gene expression while working.
Experimental tests raised larvae on identical royal jelly but capped artificial cells with either queen or worker wax; larvae under worker wax suffered higher mortality and produced smaller queens.
The team used thermal imaging, chemical and materials analyses, behavioral tracking and tracer experiments to show workers selectively gather and modify materials for royal cells.
The findings suggest social niche construction — the hive’s built environment and attendant workers — actively engineers queen development, expanding understanding of insect caste determination.







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